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1. 中北大学化工与环境学院
2. 山西大学生命科学与技术学院
Published:2007
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么兰, 韩建荣. Studies on Lipid Accumulation in Sclerotia of Penicillium sp. PT95[J]. 2007, (3): 236-240.
研究了碳氮源和无机盐对青霉PT95菌株菌核中积累类脂的影响.供试的5种碳源中
葡萄糖最有利于PT95菌株产生大量菌核
获得高产率类脂.蛋白胨、硝酸钠、硝酸铵作为氮源有利于PT95菌株菌核分化、积累类脂
尿素、氯化铵、硫酸铵不支持PT95生长.培养基中碳氮比为30∶1时
PT95菌株菌核生物量达到最高;碳氮比为70∶1时
类脂含量和类脂产率为最高.PT95菌株的菌核生物量、类脂产率随着培养基中K2HPO4浓度的增加而增加
随着FeSO4浓度的增加而减少.通过正交实验确定了有利于PT95菌株积累类脂的最佳培养基.
The effects of carbon and nitrogen sources and inorganic salts on lipid accumulation in sclerotia of Penicillium sp.PT95 were studied.Among five carbon sources tested
glucose favored the sclerotia formation and the accumulation of lipid in sclerotia of Penicillium sp.PT95.The highest lipid yield was obtained when glucose was used as carbon source.Among several nitrogen sources tested
peptone
NaNO
3
and NH_4NO
3
were favorable to the formation of sclerotia and lipid biosynthesis of this fungus.NH_4Cl and(NH
4
)_2SO
4
did not support PT95 strain growth under the tested concentrations.The results showed that lipid content and lipid yield remained the highest at a C/N-ratio of 70∶1 and sclerotial biomass remained the highest at a C/N-ratio of 30∶1.The total sclerotial biomass and lipid yield is increased with the increasing concentration of K_2HPO
4
and the decreased with the increasing conce
ntration of FeSO
4
in the media.Through orthogonal experiments
the optimum medium was obtained.
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