The electrochemical properties of myoglobin(Mb) in collagen films electrodes were investigated with cyclic voltammetry.As their biocompatibility

Collagen offers a suitable microenvironment for myoglobin to transfer electron directly with the underlying electrodes.Cyclic voltammetry of Mb-Collagen films show a pair of well-defined and nearly reversible peaks at about-0.35 V(vs.SCE)

characteristic of Mb Fe(Ⅲ)/Fe(Ⅱ) redox couple.Oxygen and hydrogen peroxide are product of electrocatalyze reduction for oxidase

and have the biological significance.They can be used to electrocatalyze reduction of oxygen and hydrogen peroxide with significant decrease of the reduction overpotential.This indicates potential value of the films for the fabrication of a new type of biosensors based on the direct electrochemistry of redox proteins.