The extraction condition of allergens from castor bean by uniform design were researched.Not only the influences of reaction ethanol concentration

temperature

time

and raw materials ratio on process

but also the best condition was obtained.SDS-PAGE was used to detect the purification of the allergen

and UV scanning

HPLC

and IR spectroscopy were used to characterize its structure and distribution.The results show that the optimal extraction condition was 85% ethanol concentration

8 h extraction time at 50 ℃ and the raw materials ratio of 1∶21(W/V).The purity of the experiment can reach 98%.